mouse interleukin 3 (mil-3 (PeproTech)
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Mouse Interleukin 3 (Mil 3, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+interleukin+3+(mil-3/rmil+4/pmc10484789-429-106-110
Average 90 stars, based on 1 article reviews
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Modification:Article Title: Real-Time Characterization of Clonal Fate Decisions in Complex Leukemia Samples by Fluorescent Genetic Barcoding. Article Snippet: Lineage negative (Lin−) bone marrow cells were purified from congenic C57BL/6Ly5.1 mice with the MojoSort Mouse Hematopoietic Progenitor Cell Isolation Kit (Biolegend, San Diego, CA, USA), according to the manufacturer’s instructions. .. Lin− cells were subsequently pre-stimulated for 2 days in basal medium (Dulbecco’s modified Eagle’s medium (DMEM; Biochrom, Berlin, Germany) with 15% (v/v) fetal bovine serum (FBS) (PanBiotech, Aidenach, Germany), 100 U/mL penicillin and 100 μg/mL streptomycin and 1 mM sodium pyruvate (PanBiotech)), supplemented with 6 ng/mL other:Article Title: A novel high-titer, bifunctional lentiviral vector for autologous hematopoietic stem cell gene therapy of sickle cell disease Article Snippet: Lin− cells were pre-stimulated at 1 × 10 6 cells/mL in Stem Cell Growth Medium (CellGenix) supplemented with mouse SCF (100 ng/mL), hTPO (100 ng/mL), mouse IL-3 (mIL-3) (20 ng/mL), and Article Title: A somatic mutation in moesin drives progression into acute myeloid leukemia Article Snippet: Cells were cultured in StemSpan serum-free expansion media (STEMCELL Technologies) supplemented with gentamicin (50 ng/ml; Invitrogen), 100 μM 2-mercaptoethanol (Thermo Fisher Scientific), mouse Interleukin-3 (mIL-3) (5 ng/ml), human Flt3-Ligand (hFlt3) (20 ng/ml), mouse thrombopoietin (mTPO) (10 ng/ml), Article Title: Heme oxygenase-1 protects cells from replication stress. Article Snippet: HSC culture medium was StemSpan SFEM (Stem Cell Technologies), supplemented with 20 % of BIT 9500 Serum Substitute (Stem Cell Technologies), Article Title: ETV6-NCOA2 fusion induces T/myeloid mixed-phenotype leukemia through transformation of nonthymic hematopoietic progenitor cells Article Snippet: Enriched cells were cultured in RPMI supplemented with mouse interleukin-3 (mIL-3) 10 ng/mL, mIL-6 10 ng/mL, Article Title: Heme oxygenase-1 protects cells from replication stress Article Snippet: HSC culture medium was StemSpan SFEM (Stem Cell Technologies), supplemented with 20 % of BIT 9500 Serum Substitute (Stem Cell Technologies), Retroviral:Article Title: Defective ribosome assembly impairs leukemia progression in a murine model of acute myeloid leukemia. Article Snippet: Article Transduction:Article Title: Defective ribosome assembly impairs leukemia progression in a murine model of acute myeloid leukemia. Article Snippet: Article Cell Culture:Article Title: Defective ribosome assembly impairs leukemia progression in a murine model of acute myeloid leukemia. Article Snippet: Article Article Title: Single-cell multi-omics identifies chronic inflammation as a driver of TP53 -mutant leukemic evolution Article Snippet: BM LSK cell cycle was assessed by flow cytometry using Ki-67 and DAPI staining, after fixation and permeabilization (BD Cytofix/Cytoperm and Permeabilization Buffer Plus, BD Biosciences). .. Fifty CD45.1 ( Trp53 R172H/+ ) or CD45.2 (WT) LSK (Lin - Sca1 + c-Kit + ) cells from poly(I:C)-treated and control recipient mice were sorted and cultured for 1 week into Complete X-vivo15 media (BE-04-418Q, Lonza) supplemented with 10% FCS (Sigma-Aldrich, F9665), 0.1 mM 2-mercaptoethanol (Gibco, 21985023), 1% penicillin-streptomycin (PAA laboratories), 2 ng ml −1 mouse stem cell factor (mSCF; PeproTech, 250-03), 10 ng ml −1 mouse granulocyte–monocyte colony-stimulating factor (mGM–CSF; Immunex), 5 ng ml −1 human thrombopoietin (hTPO; PeproTech, 300-18-10), 10 ng ml −1 human granulocyte colony-stimulating factor (hG-CSF; Neopogen) 5 ng ml −1 human FLT3 ligand (hFL; Immunex, 300-19), 5 ng ml −1 Control:Article Title: Single-cell multi-omics identifies chronic inflammation as a driver of TP53 -mutant leukemic evolution Article Snippet: BM LSK cell cycle was assessed by flow cytometry using Ki-67 and DAPI staining, after fixation and permeabilization (BD Cytofix/Cytoperm and Permeabilization Buffer Plus, BD Biosciences). .. Fifty CD45.1 ( Trp53 R172H/+ ) or CD45.2 (WT) LSK (Lin - Sca1 + c-Kit + ) cells from poly(I:C)-treated and control recipient mice were sorted and cultured for 1 week into Complete X-vivo15 media (BE-04-418Q, Lonza) supplemented with 10% FCS (Sigma-Aldrich, F9665), 0.1 mM 2-mercaptoethanol (Gibco, 21985023), 1% penicillin-streptomycin (PAA laboratories), 2 ng ml −1 mouse stem cell factor (mSCF; PeproTech, 250-03), 10 ng ml −1 mouse granulocyte–monocyte colony-stimulating factor (mGM–CSF; Immunex), 5 ng ml −1 human thrombopoietin (hTPO; PeproTech, 300-18-10), 10 ng ml −1 human granulocyte colony-stimulating factor (hG-CSF; Neopogen) 5 ng ml −1 human FLT3 ligand (hFL; Immunex, 300-19), 5 ng ml −1 |
