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mouse interleukin 3 (mil-3  (PeproTech)


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    Structured Review

    PeproTech mouse interleukin 3 (mil-3
    Mouse Interleukin 3 (Mil 3, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+interleukin+3+(mil-3/rmil+4/pmc10484789-429-106-110
    Average 90 stars, based on 1 article reviews
    mouse interleukin 3 (mil-3 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Modification:

    Article Title: Real-Time Characterization of Clonal Fate Decisions in Complex Leukemia Samples by Fluorescent Genetic Barcoding.
    Article Snippet: Lineage negative (Lin−) bone marrow cells were purified from congenic C57BL/6Ly5.1 mice with the MojoSort Mouse Hematopoietic Progenitor Cell Isolation Kit (Biolegend, San Diego, CA, USA), according to the manufacturer’s instructions. .. Lin− cells were subsequently pre-stimulated for 2 days in basal medium (Dulbecco’s modified Eagle’s medium (DMEM; Biochrom, Berlin, Germany) with 15% (v/v) fetal bovine serum (FBS) (PanBiotech, Aidenach, Germany), 100 U/mL penicillin and 100 μg/mL streptomycin and 1 mM sodium pyruvate (PanBiotech)), supplemented with 6 ng/mL murine interleukin 3 (mIL-3), 10 ng/mL human interleukin 6 (hIL-6) and 20 ng/mL murine stem cell factor (mSCF) (=36SF medium; all cytokines from PeproTech GmbH, Hamburg, Germany). .. Afterwards, 5 × 104 cells were seeded into 96 well round bottom plates with 100 µL 36SF medium supplemented with 4 µg/mL protamine sulfate for overnight transduction with a VSVg-pseudotyped gammaretroviral vector (pRSF91-Meis1-2A-Hoxa9.i2.Puro.LVpre) expressing murine cDNAs for Meis1, Hoxa9 and the puromycin-resistance (pac) gene [20].

    other:

    Article Title: A novel high-titer, bifunctional lentiviral vector for autologous hematopoietic stem cell gene therapy of sickle cell disease
    Article Snippet: Lin− cells were pre-stimulated at 1 × 10 6 cells/mL in Stem Cell Growth Medium (CellGenix) supplemented with mouse SCF (100 ng/mL), hTPO (100 ng/mL), mouse IL-3 (mIL-3) (20 ng/mL), and hFlt3-L (100 ng/mL), all from Peprotech.

    Article Title: A somatic mutation in moesin drives progression into acute myeloid leukemia
    Article Snippet: Cells were cultured in StemSpan serum-free expansion media (STEMCELL Technologies) supplemented with gentamicin (50 ng/ml; Invitrogen), 100 μM 2-mercaptoethanol (Thermo Fisher Scientific), mouse Interleukin-3 (mIL-3) (5 ng/ml), human Flt3-Ligand (hFlt3) (20 ng/ml), mouse thrombopoietin (mTPO) (10 ng/ml), mouse stem cell factor (mSCF) (50 ng/ml; all from PeproTech), and doxycycline (1 μg/ml; Sigma-Aldrich).

    Article Title: Heme oxygenase-1 protects cells from replication stress.
    Article Snippet: HSC culture medium was StemSpan SFEM (Stem Cell Technologies), supplemented with 20 % of BIT 9500 Serum Substitute (Stem Cell Technologies), murine stem cell factor (mSCF, Peprotech), human thrombopoietin (hTPO, Peprotech), murine interleukin-3 (mIL-3, Peprotech), and human erythropoietin (hEPO, Sigma-Aldrich), all at a concentration of 20 ng/mL.

    Article Title: ETV6-NCOA2 fusion induces T/myeloid mixed-phenotype leukemia through transformation of nonthymic hematopoietic progenitor cells
    Article Snippet: Enriched cells were cultured in RPMI supplemented with mouse interleukin-3 (mIL-3) 10 ng/mL, mIL-6 10 ng/mL, mouse stem cell factor (mSCF) 50 ng/mL (PeproTech), and cortisol 0.1 μM (Sigma).

    Article Title: Heme oxygenase-1 protects cells from replication stress
    Article Snippet: HSC culture medium was StemSpan SFEM (Stem Cell Technologies), supplemented with 20 % of BIT 9500 Serum Substitute (Stem Cell Technologies), murine stem cell factor (mSCF, Peprotech), human thrombopoietin (hTPO, Peprotech), murine interleukin-3 (mIL-3, Peprotech), and human erythropoietin (hEPO, Sigma-Aldrich), all at a concentration of 20 ng/mL.

    Retroviral:


    Transduction:


    Cell Culture:


    Article Title: Single-cell multi-omics identifies chronic inflammation as a driver of TP53 -mutant leukemic evolution
    Article Snippet: BM LSK cell cycle was assessed by flow cytometry using Ki-67 and DAPI staining, after fixation and permeabilization (BD Cytofix/Cytoperm and Permeabilization Buffer Plus, BD Biosciences). .. Fifty CD45.1 ( Trp53 R172H/+ ) or CD45.2 (WT) LSK (Lin - Sca1 + c-Kit + ) cells from poly(I:C)-treated and control recipient mice were sorted and cultured for 1 week into Complete X-vivo15 media (BE-04-418Q, Lonza) supplemented with 10% FCS (Sigma-Aldrich, F9665), 0.1 mM 2-mercaptoethanol (Gibco, 21985023), 1% penicillin-streptomycin (PAA laboratories), 2 ng ml −1 mouse stem cell factor (mSCF; PeproTech, 250-03), 10 ng ml −1 mouse granulocyte–monocyte colony-stimulating factor (mGM–CSF; Immunex), 5 ng ml −1 human thrombopoietin (hTPO; PeproTech, 300-18-10), 10 ng ml −1 human granulocyte colony-stimulating factor (hG-CSF; Neopogen) 5 ng ml −1 human FLT3 ligand (hFL; Immunex, 300-19), 5 ng ml −1 mouse interleukin 3 (mIL-3; PeproTech, 213-13). ..

    Control:

    Article Title: Single-cell multi-omics identifies chronic inflammation as a driver of TP53 -mutant leukemic evolution
    Article Snippet: BM LSK cell cycle was assessed by flow cytometry using Ki-67 and DAPI staining, after fixation and permeabilization (BD Cytofix/Cytoperm and Permeabilization Buffer Plus, BD Biosciences). .. Fifty CD45.1 ( Trp53 R172H/+ ) or CD45.2 (WT) LSK (Lin - Sca1 + c-Kit + ) cells from poly(I:C)-treated and control recipient mice were sorted and cultured for 1 week into Complete X-vivo15 media (BE-04-418Q, Lonza) supplemented with 10% FCS (Sigma-Aldrich, F9665), 0.1 mM 2-mercaptoethanol (Gibco, 21985023), 1% penicillin-streptomycin (PAA laboratories), 2 ng ml −1 mouse stem cell factor (mSCF; PeproTech, 250-03), 10 ng ml −1 mouse granulocyte–monocyte colony-stimulating factor (mGM–CSF; Immunex), 5 ng ml −1 human thrombopoietin (hTPO; PeproTech, 300-18-10), 10 ng ml −1 human granulocyte colony-stimulating factor (hG-CSF; Neopogen) 5 ng ml −1 human FLT3 ligand (hFL; Immunex, 300-19), 5 ng ml −1 mouse interleukin 3 (mIL-3; PeproTech, 213-13). ..



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    Image Search Results


    ETV6-NCOA2 is a T-cell oncogene that induces immature T-cell arrest in murine BM progenitors. (A) Schematic representation of ETV6-NCOA2 (EN2) fusion. bHLH-PAS, basic helix-loop-helix Per-ARNT-SIM domain; NID, nuclear receptor interaction domain. (B) Murine BM progenitors treated with fluorouracil were transduced with MSCV-MIGR1-IRES-GFP retroviruses expressing ETV6-NCOA2, empty vector, or KAT6A-NCOA2 (KN2) and plated in methylcellulose (IL-3, IL-6, and SCF). Colonies were counted and re-plated every 7 to 10 days. EN2 induces self-renewal of transduced cells on methylcellulose culture compared with the empty vector–transduced cells in the second and third replate (Mann-Whitney U test P = .014) (n = 3). (C) Lineage-negative cells (lin–) were enriched from wt-C57BL/6 mice; the cells were transduced with EN2, KN2, or empty vector, incubated in liquid culture (IL-3, IL-6, and SCF) for 5 days, and sorted for GFP+. The RNA of the GFP+ sorted cells was sent for bulk RNA-seq. Gene set enrichment analysis (GSEA) of the EN2 vs empty vector–transduced cells demonstrated enrichment of Notch1 signature (NES, 1.67; FDR, 0.011), ETP (NES, 1.98; FDR, 0.006), and early thymic signature (NES, 2.65; FDR, 0.0). (D) Murine BM HSPCs treated with fluorouracil were transduced with either EN2 or empty vector and plated on OP9-DL4 stroma (IL-7, Flt3L) for 3 weeks and then immunophenotyped by flow cytometry (n = 4). Left panel: average immunophenotype results (Mann-Whitney U test for EN2 vs empty vector P = .02 in DN1, DN2, and DN3). Right panel: representative example of CD44 and CD25 flow cytometry results. AF700, Alexa Fluor 700; HSC, hematopoietic stem cell; SS, side scatter.

    Journal: Blood

    Article Title: ETV6-NCOA2 fusion induces T/myeloid mixed-phenotype leukemia through transformation of nonthymic hematopoietic progenitor cells

    doi: 10.1182/blood.2020010405

    Figure Lengend Snippet: ETV6-NCOA2 is a T-cell oncogene that induces immature T-cell arrest in murine BM progenitors. (A) Schematic representation of ETV6-NCOA2 (EN2) fusion. bHLH-PAS, basic helix-loop-helix Per-ARNT-SIM domain; NID, nuclear receptor interaction domain. (B) Murine BM progenitors treated with fluorouracil were transduced with MSCV-MIGR1-IRES-GFP retroviruses expressing ETV6-NCOA2, empty vector, or KAT6A-NCOA2 (KN2) and plated in methylcellulose (IL-3, IL-6, and SCF). Colonies were counted and re-plated every 7 to 10 days. EN2 induces self-renewal of transduced cells on methylcellulose culture compared with the empty vector–transduced cells in the second and third replate (Mann-Whitney U test P = .014) (n = 3). (C) Lineage-negative cells (lin–) were enriched from wt-C57BL/6 mice; the cells were transduced with EN2, KN2, or empty vector, incubated in liquid culture (IL-3, IL-6, and SCF) for 5 days, and sorted for GFP+. The RNA of the GFP+ sorted cells was sent for bulk RNA-seq. Gene set enrichment analysis (GSEA) of the EN2 vs empty vector–transduced cells demonstrated enrichment of Notch1 signature (NES, 1.67; FDR, 0.011), ETP (NES, 1.98; FDR, 0.006), and early thymic signature (NES, 2.65; FDR, 0.0). (D) Murine BM HSPCs treated with fluorouracil were transduced with either EN2 or empty vector and plated on OP9-DL4 stroma (IL-7, Flt3L) for 3 weeks and then immunophenotyped by flow cytometry (n = 4). Left panel: average immunophenotype results (Mann-Whitney U test for EN2 vs empty vector P = .02 in DN1, DN2, and DN3). Right panel: representative example of CD44 and CD25 flow cytometry results. AF700, Alexa Fluor 700; HSC, hematopoietic stem cell; SS, side scatter.

    Article Snippet: Enriched cells were cultured in RPMI supplemented with mouse interleukin-3 (mIL-3) 10 ng/mL, mIL-6 10 ng/mL, mouse stem cell factor (mSCF) 50 ng/mL (PeproTech), and cortisol 0.1 µM (Sigma).

    Techniques: Transduction, Expressing, Plasmid Preparation, MANN-WHITNEY, Incubation, RNA Sequencing Assay, Flow Cytometry